Analysis of endothelial cells in vitro in a 3-dimensional ECM showed that the PAK peptide markedly blocked forma- tion of extended multicellular structures without apparent deleterious effects on single cell morphology or number. Previous studies showed that these structures contain lumens and therefore resemble capillary tubes in vivo.
The effect of the PAK peptide is therefore consistent with inhibition of cell migration. Analysis of angiogenesis in a chick CAM model also revealed significant inhibition by the PAK peptide.
上記の文献によると: ”The PAK and control peptides were synthesized by the Scripps peptide synthesis facility.” 「PAKおよびコントロールペプチドは、スクリップスペプチド合成施設によって合成しました。」 と書かれていましたから、このPAK ペプチドはPAKタンパク質にくっつくように人工的に合成したものでしょう。